(P34) Investigation of the Protective Effect of Dexpanthenol on Experimental Liver Ischemia Reperfusion Injury in Rats

Friday, September 8, 2023

12:30 PM - 1:30 PM EEST

Poster Presenter / Primary Author(s)

AS

Altan Sipahi, MD

surgeon
TURKISH SURGICAL ASSOCIATION
Eskisehir, Eskisehir, Turkey

Co-Author(s)

AS

Adnan Sahin, MD

professor
TURKISH SURGICAL SOCIETY
eskisehir, Eskisehir, Turkey
NE

Nilufer Erkasap, MD

Professor
Eskisehir Osmangazi University Medical Faculty
Eskisehir, Eskisehir, Turkey

Objectives: The aim of this study is to investigate the protective effect of dexpanthenol applied before and during ischemia on ischemia and reperfusion injury in the rats.

Background: Ischemia Reperfusion (I / R) damage can happen after hypovolemic shock, chronic liver disease, major tumor resections, surgical intervention to hepatic trauma, vascular reconstructions, and dextro-transplantation. Dexpanthenol is an alcohol derivative of pantothenic acid. It turns into D- pantothenic acid in the liver.

Methods: The study aims to determine ischemia effects and reperfusion effect of dexpanthenol agent by forming 3 groups of 30 Wistar Albino type male 200-250 gr rats. In group 1, liver tissue sample was planned collected without ischemia. In the second group, 45 minutes of ischemia was planned, 30 minutes before ischemia and just before reperfusion, intraperitoneal serum physiologic application was planned. In Group 3, it was planned to administer 500mg / kg dexpanthenol intraperitoneally 30 minutes before ischemia and just before reperfusion. Serum and tissue samples were planned to be collected in the 45th minute following reperfusion in groups 2 and 3. Following the procedure, sacrification was planned with cervical dislocation.

Results: AST and ALT compared to the control group in the sham group (p < 0.05), p value for AST and ALT compared to the sham group in the experimental group was significantky decreased (p < 0.01) There was no significant difference in 3 groups for p value for ALP (p < 0.01). Plasma TNF-a IL-1 and IL-6 levels of the dexpantenol group were found to be statistically significantly lower than the levels of the sham group (p < 0.01). As a result, liver dysfunction in the liver ischemia reperfusion model and liver function tests after dexpanthenol treatment (IL-1, IL-6 and TNF-a) has been observed that partial improvement and decreases in oxidative stress parameters, but larger studies are required to determine its effectiveness in primary I / R damage.

Conclusions:

References (Optional):

Funding (Optional): Eskisehir Osmangazi University Faculty of Medicine